THE STUDY OF URINE SPOT SAMPLES DRIED ON THE CHEESECLOTH USING METHODS OF MOLECULAR GENETICS
Journal Title: Молодий вчений - Year 2017, Vol 3, Issue 43
Abstract
In our study, we have investigated the amount of DNA, isolated from experi-mental urine samples, kept at the same temperature during the different periods of time, and the compatibility of isolated DNA for the further typing via PCR; also the quality of obtained DNA profiles was evaluated. The results have demonstrated the absence of significant difference (P> 0,05) in the quantity of isolated DNA on the 1, 30, 60 and 90 day. As was shown in our study, the degradation process in the urine samples kept at room temperature (22°C) manifests itself by gradual decrease of amount of the obtained DNA, on average, by 2,5 % in women and by 3,5 % in men. Thus, this is theoretically possible to obtain the amount of DNA sufficient for the further genetic typing from the spot of urine 2,0 x 2,0 cm containing 3,0 ml of urine, on the 90th day. On the basis of conclusions of our investigation we have proposed an algorithm of realization of forensic medical examination of urine samples dried on the cheesecloth using the methods of molecular genetics.
Authors and Affiliations
R. G. Kryvda
Keywords
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