Total saponins in Rubus parvifolius L. induce lymphoma cells apoptosis through upregulated Bax/Fas and downregulated Bcl-2 in vivo and in vitro
Journal Title: Traditional Medicine Research - Year 2019, Vol 4, Issue 2
Abstract
Purpose: To investigate the effect of total saponins of Rubus parvifolius L. (TSRP) on lymphoma Raji cells and further discuss its mechanism. Methods: The model of nude mice bearing Raji cells was established, the volume, weight and inhibition rate of the transplanted tumor were analyzed and compared after different concentrations of TSRP treatment. Cell apoptosis and expression of Bcl-2, Bax, Fas proteins were detected by TUNEL and immunohistochemiscal method respectively. Effects of TSRP on cell proliferation were tested with MTT assay in vitro. Cell apoptosis and expression of Caspase-9, Caspase-3, Bcl-2, Bax and Fas proteins were tested with DAPI staining and Western blot. Results: TSRP significantly reduced the volume and tumor weight of Raji subcutaneous transplanted tumor and induced the apoptosis of Raji cells in vivo. The tumor inhibition rate of high-dose (100 mg/kg) TSRP is 90.84%. The TUNEL test results show that the fluorescence intensity of the tumor issue treated with TSRP is significantly improved. Compared with the control group, the fluorescence intensity of high-concentration TSRP is 82.43 ± 7.81, which is significantly different (P < 0.001). The results of immunohistochemistry test showed that the Bcl-2 expression of Raji cell treated with TSRP is obviously reduced, and Bax expression is obviously increased. Meanwhile, compared with that of control group, Fas expression is obviously reduced. MTT assay showed that TSRP can significantly inhibit proliferation of Raji cells with dose dependence. The inhibition rate of 400 μg/mL TSRP is 53.46 ± 4.90% (P < 0.001). DAPI staining results showed that TSRP can significantly induce cell apoptosis. According to Western blot results, it is found that TSRP can significantly inhibit activity of Bcl-2 and increase Bax expression, and TSRP can also inhibit Fas expression. Meanwhile, expression of Caspase-9 and Caspase-3 is also increased. Conclusion: TSRP could inhibit the proliferation of lymphoma via induction of apoptosis in a time and dose-dependent manner. Apoptotic signaling induced by TSRP was characterized by up-regulating Bax, Fas and Caspase-8 protein expression, and down-regulating of Bcl-2 protein expression.
Authors and Affiliations
Xiao-Feng Xu,Ru-Bin Cheng,Xue-Jin Zhang,Rui-Lan Gao
Keywords
Related Articles
- EP ID EP45112
- DOI 10.12032/TMR20190304108
- Views 361
- Downloads 0
Stir up a fire and burn oneself: the secret of fire therapy
On December 25, 2018, Ding Xiang Yuan Weixin Public Platform, a well-known medical academic exchange platform in China, published an article "The Ten-Billion Health Care Empire Quan Jian and the Chinese Families in its S...
A Literature Review of the Acupoint Plaster Therapy for Asthma in Summer
Treating winter diseases in summer is characterized by warming Yang, which is recuperating in summer, to treat some chronic diseases commonly seen in winter. This treatment belongs to the chronomedicine of Traditional Ch...
A network pharmacology approach to investigate the mechanisms of Si-Jun-Zi decoction in the treatment of gastric precancerous lesions
Objective: To find out the potential mechanisms of Si-Jun-Zi (SJZ) decoction in the treatment of gastric precancerous lesions (GPL). Methods: A network pharmacology approach was used to analyze the active compounds, drug...
Epigenetic regulations of hematopoietic stem cells ageing and the regulation of traditional Chinese medicine
The current progress in the study of DNA methylation and histone modifications regulating HSCs ageing and the epigenetic role of TCM in the treatment of hematological malignancies were summarized. Age-related defects...
Systematic analysis of components and contents in Houttuynia cordata Thunb
Objective: This study aims to provide the material basis for the molecular mechanism of Yuxingcao (Houttuynia cordata Thunb, HCT) through the method of evidence-based study to summarize the natural constituents isolated...