Вплив наночастинок Кремнію на криву зростання бактерій в присутності гумінових речовин у рідкому живильному середовищі.
Journal Title: Питання біоіндикації та екології - Year 2017, Vol 22, Issue 2
Abstract
Silicon (Si) is an element that attracts the attention of scientists around the world. Nanoparticles of silicon are actively investigating as a carrier for protein molecules, since they have a large active surface, chemically and thermally stable. Nanoparticles of silicon that are smaller than 5 nm are received by pulsed laser ablation. Electronic properties of nanoparticles of silicon allow to use them as photoluminescent labels for photodynamic diagnosis and therapy. Nanoparticles of Si increase the permeability of cell membranes, making the drugs connected to particles necessity fewer. Silicon is also a good preservative, its particles hinder the development of pathogens in food. Nanoparticles of silicon adsorbed on the cell wall of prokaryotes, lowered affinity for dyes, led to polymorphism and had moderate bactericidal effect. Humic substances (HS) are a source of nutrition elements, growth factors, enzymes, vitamins and other biologically active substances required for plant growth and development, as well as to enhance their protective function to the action of adverse factors. That is why the aim of the study was to investigate the physical and chemical properties of HS and to conduct a comparative analysis of growth curves probiotic culture with the addition of nanoparticles of Si and HS. The object of the research was a bacterial culture of Bacillus subtilis, which we were provided from a collection of museum culture D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine. Physical and chemical parameters (ORP, pH and tºC) of humic substances are measured with the help of device ORP/pH meter «MP-103». We were using 1% aqueous solution of sodium salts of humic acids of peat – «Huminat» in experiments. Colloidal Si solution was provided to us by the I. M. Frantsevich Institute for Problems of Materials Sciences. In order to create the allocation of water, the fulvic transitional peat was taken from Maschanskoho Tracts area Kostopil district Rivne region. For extraction of peat FA the gravimetric method of Erdmann was used. Optical density was measured on photoelectric calorimeter CPK-2 at a wavelength of 540 nm. Stimulatory effect of huminat on the growth and reproduction of bacteria Bacillus subtilis was noted. Most intensive cultural growth observed in the stationary phase of growth for huminat 3 ml, the optical density of the cells was 0.51 c. u., the lowest growth recorded in the control cultures (0.35 c. u.). By adding nanoparticles of Si we noted decrease of cell growth culture, namely with increasing concentrations of nanoparticles of silicon, optical density decreases, even in the presence and water extraction of peat. However, the concentration of silicon 0.5 ml (and huminat 2 ml) had the ability to raise growth of Bacillus subtilis. D index was 0.67 c. u., which is 1.3 times more than the control. Thus, the nanoparticles of Si possess not only a strong pharmacological activity but also toxicity. It is therefore necessary to carry out advanced research for the safe use of nanoparticles of silicon in medicine, pharmacology and pharmacy. Результаты исследования продемонстрировали стимулирующее действие препарата «Гуминат» на рост и размножение бактерий Bacillus subtilis. Наиболее интенсивный рост культуры наблюдался в стационарной фазе роста в присутствии 3 мл гумината, оптическая плотность клеток составляла 0,51 у. е., наименьший рост культуры зафиксирован в контроле (0,35 у. е.). С увеличением концентрации наночастиц Кремния наблюдалось уменьшение роста культуры, даже в присутствии гумината и водной витяжки из торфа. Концентрация Кремния 0,5 мл (в присутствии 2 мл гумината), наоборот, стимулировала рост сенной палочки. Таким образом, наночастицы Кремния обладают не только выраженной фармакологической активностью, но и токсичностью.
Authors and Affiliations
Рильський О. Ф. , Крупєй К. С. , Волошина О. М. , Сорокина Д. Р. , Сорокина О. Р. Rylsky O. F. , Krupey К. S. , Voloshina O. M. , Sorokina D. R. , Sorokina O. R.
Keywords
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