Analytical method development and validation of Artesunate and Amodiaquine hydrochloride in tablet dosage form by RP-HPLC
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination of Artesunate (ART) and Amodiaquine hydrochloride (AMO) in pharmaceutical dosage form. The column used was inertsil ODS C18 (250*4.6mm,5µ) in isocratic mode, with mobile phase containing phosphate buffer- acetonitrile-methanol (50:30:20) adjusted to pH 5.8 using ortho phosphoric acid was used and injection volume of 20µL, with a flow rate of 1.0ml/min. and effluents were monitored at 208 nm. The retention times of artesunate and amodiaquine hydrochloride were 5.03 min and 2.77 min, respectively. The linearity for Artesunate and Amodiaquine hydrochloride were in the range of 15-35 mcg/ml and 45.9-107.1 mcg/ml respectively with correlation coefficient of r2=0.999 for both.The assay of the proposed method was found to be 98.56% and 99.08%. The recoveries of artesunate and amodiaquine hydrochloride were found to be 99.81% and 99.3%, respectively. The % RSD from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of artesunate and amodiaquine hydrochloride in bulk and in Pharmaceutical dosage form.
Authors and Affiliations
P RajaRao, Nanda Kishore Agarwal
Keywords
Related Articles
- EP ID EP33161
- DOI -
- Views 347
- Downloads 0
Investigation of physiochemical and vegetative properties of two barley lines
Physiochemical and vegetative properties of two barely lines in “EBYT88-17 AND EBTY88-20” purchased from agriculture center of Golestan Province, Iran were investigated for enzyme activity, sprouting energy and suitabil...
A Study on prescribing and cost pattern of antimicrobial agents in a tertiary care teaching hospital
Now-a-days many drugs are showing resistance followed by ineffective treatment. It has become a major obstacle in treatment. Among many drugs antimicrobials drugs are also highly resistance, Anti-Microbial Resistance (...
FORMULATION, OPTIMIZATION AND IN-VITRO EVALUATION OF NIFEDIPINE SUSTAINED RELEASE TABLET
The objective of the present study was to develop sustained release tablet of Nifedipine. The drug is a dihydropyridine derivative mainly indicated for the treatment of Hypertension. The drug belongs to the therapeutic...
SCREENING OF BIOACTIVE ACTINOMYCETES FROM SUGARCANE SOILS
Actinomycetes and Streptomyces are once considered to be microbiological curiosities of no great economic importance, but have become the subjects of intensive searches for source of new biologically active compounds....
A review on antidepressant activity of Agomelatine
Agomelatine, a melatonergic antidepressant with a rapid onset of action, is one of the most recent drugs in the antidepressant category. Agomelatine’s antidepressant actions are attributed to its sleeppromoting and chro...