Біомаркерні показники селезінки Rana ridibunda P. (BIOMARKERS INDICATORS OF RANA RIDIBUNDA P.)
Journal Title: Питання біоіндикації та екології - Year 2018, Vol 23, Issue 1
Abstract
Marsh frogs are exposed to the factors of an environment in the process of their ontogenesis, so may be indicators of the presence of violations in ecotopes. The purpose of our study was to investigate the morphological parameters of the spleen in R. ridibunda P. for the proposition of markers in the system of biomonitoring. The animals were selected to study according to the principle of analogues by age (2–3 years), the sex, the mass of the class Amphibia – Amphibians Rana ridibunda P., 1771 – marsh frog, n = 254. The linear parameters of the spleen and its relative mass were determined. For histological study, the material was fixed in a 10–12% solution of formalin, the histological cuts were stained with Ehrlich hematoxylin and eosin, using the Van Gison`s method. For cytological examination of the spleen, the specimens were fixed with 2.5% glutaraldehyde solution, contrasted with 2 % solution of uranyl acetate and lead citrate and examined by an electron microscope of PEM-125K for an increase in 4–20 thousand times. The length of the spleen was 5.50±1.87 mm; the width – 4.12±1.72 mm; the height – 3.39±1.66 mm. The form was defined as round-oval. Anatomical studies of abnormal deviations in the form of an organ were not found. The relative mass is 0.1170±0.0215 %. In the mature frogs, the histoarchitectonics of the spleen are completely formed. According to the histological analysis, the musculoskeletal system and parenchyma were clearly differentiated. In the pulp, there were elongated shapes of trabecula, among which the vascular tissue dominated over the connective. The relative area of the musculoskeletal system was 5.39±0.07 % of the total area of the spleen. The red pulp occupied a significant relative area of the spleen – 80.67±6.53 %. The predecessor of red blood cells, red blood cells, erythroblast, adventitious cells, platelets, myeloblasts, myelocytes were differentiated among the cytological population. A large number of lymphocytes and dense cell placement, compared to red, was a characteristic feature of white pulp. Plots of white pulp were located in the spleen irregularly and had an irregular shape. Its composition revealed lymphoid nodules and lymphoid sheaths near the vessels. The relative share of white pulp equalled 15.36±5.71 %. The ratio of the support-contractive apparatus and the pulp equalled 1:24.19, the ratio of white pulp and red pulp equalled 1:5.25. The peculiarity of the cell population of the spleen of the frogs is the availability of melanomacrophagal clusters with a total area of 1.82±0.42 %. З метою біомоніторингу оточуючого середовища раціонально застосовувати амфібії. Доцільною є розробка маркерних показників селезінки, яка є дуже чутливою до змін параметрів довкілля. Вивчені головні анатомічні, гістологічні, цитологічні показники селезінки представника класу Амфібії – жаби озерної. Встановлені морфологічні особливості селезінки: наявність меланомакрофагальних скупчень, відсутність чіткої межі між білою і червоною пульпами, наявність пульпарних трабекул з домінуванням серед них судинних, улімфоїдних вузликах дифференціювалась одна периартеріальна зона. Визначенівідносна маса, відносні площі основних структур, співвідношення між ними, характеристики лімфоцитів, еритроцитів як основні тестові критерії.
Authors and Affiliations
О. Ф. Дунаєвська (Oksana Dunaievska)
Keywords
Related Articles
- EP ID EP507965
- DOI 10.26661/2312-2056/2018-23/1-14
- Views 109
- Downloads 0
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