Comparison of concentration technique for detection of coccidian parasites among HIV seropositive patients and its correlation with CD4 T cell count
Journal Title: Medpulse International Journal of Microbiology - Year 2017, Vol 1, Issue 2
Abstract
Aim and Objectives: To study the prevalence of coccidian parasites among HIV seropositive patients and to compare concentration techniques for detection of these parasites and also to evaluate the association between CD4 T cell count and presence of coccidian parasites. Material and Method: Stool specimens were collected in wide mouthed, clean, dry, plastic containers with tight fittings lids from 510 patients who were HIV seropositive with or without diarrhoea. Microscopic examination was done by direct saline and iodine mount. All the specimens were subjected to concentration techniques for detection of coccidian parasites. About 5 ml of venous blood was collected from all patients in a vacutainer containing K2 EDTA for estimation of CD4/CD8 count. Result and Observations: Out of 510 patients, 161 (31.57%) had CD4 T cell count less than 200 cell/ µl. The maximum incidence of diarrhoea was seen among patients with CD4 T cell count less than 200 cells/ µl. A total of 67 (13.33 %) coccidian parasites were detected by formol ether sedimentation technique, While 78 (15.29%) were detected by Sheather’s sucrose floatation technique. Detection rate of intestinal parasite was more in patients presented with diarrhoea having CD4 T cell count less than 200 cells/µl. Conclusion: Detection rate of coccidian parasite was more by concentration technique as compared to direct saline mount and detection of coccidian parasites by formol ether centrifugal sedimentation technique followed by modified Z-N staining is as effective as Sheather’s sucrose floatation technique. CD4 T cell count has been an excellent indicator which defines the degree of immune suppression.
Authors and Affiliations
V V Chincholkar, N V Abdagire, S L Nilekar, S R More
Keywords
Related Articles
- EP ID EP180579
- DOI -
- Views 96
- Downloads 0
Invasive candidiasis outbreak in a neonatal intensive care unit (NICU), a descriptive study from tertiary care teaching hospital of western part of Maharashtra
Background: Candidemia outbreaks are frequent event take place in NICU accounting about 9-13%. Especially non-albicans Candida species emerged as an important cause of various outbreaks in NICU. There are various predisp...
Prevalence of infection by Acinetobacter species and their antibiogram at a tertiary care hospital
Background: Acinetobacter species have emerged as an important nosocomial pathogen involved in outbreaks of hospital infections. Acinetobacter species tend to be resistant to a variety of antibiotics and thus the infecti...
Detection of prevalence of antinuclear antibodies in clinically suspected cases of systemic autoimmune diseases by indirect immunofluorescence test in a tertiary care hospital
Background: Systemic autoimmune diseases (SAD) are the diseases where multiple organs are involved in the presence of auto antibodies directed against sub-cellular structures or molecules and are characterized by presenc...
Chlamydial infection association with syphilis in patients attending STD clinic in a tertiary care hospital
Background: Co-infection of Chlamydia with syphilis highlights the importance of early laboratory diagnosis and specific treatment. It is worthwhile to screen patients for this infection and to initiate prompt treatment...
Study on antibiotic resistance pattern of MRSA among patients in M.G.M. Hospital
Problem statement: The bacterium S. aureus is a common cause of human infection, and it is becoming increasingly virulent and resistant to antibiotics. Our understanding of the evolution of this species has been greatly...