Effect of Human Umbilical Cord Blood Stem Cells on Hepatic Regeneration and Immune Response in Liver Fibrosis in Rats
Journal Title: Journal of Physiology and Pharmacology Advances - Year 2015, Vol 5, Issue 2
Abstract
To study the effect CD34+, CD34- and mononuclear cells in a rat model of liver fibrosis, 65 adult female albino rats were divided into 5 equal groups. Group I served as control, while hepatic fibrosis was induced in the other 4 groups by subcutaneous injection of CCl4 for 8 weeks. Group II did not receive any treatment while groups III, IV and V were treated by CD34+ stem cells, CD34- fraction, or mononuclear cells, respectively, as a single IV injection. Liver function and fibrosis were assessed by serum levels of AST, ALT, GGT, albumin and procollagen III. At the end of the experiments the liver was extracted for immunohistochemical (IHC) detection of homing and immune-response markers, as well as for histopathological study. There was a significant improvement of serum levels of AST, ALT and GGT in the 3 treated groups, whereas serum albumin and procollagen III did not show significant change. Histopathology showed improvement in hepatic damage in the stem cell treated rats. IHC staining showed positive staining for cytokeratin 19, human CD34 and human albumin. IHC staining with monoclonal mouse anti-rat CD68, CD8 and CD4 showed no differences among the 3 groups treated with stem cells. We conclude that HUCB stem cells were transdifferentiated into hepatocytes when transplanted in rats with an injured liver and improved liver functional and structural alterations without stimulating a significant immune response.
Authors and Affiliations
K. E. Ismail , S. M. Greish , E. A. Elmo’men , Y. M. El-Wazir
Keywords
Related Articles
- EP ID EP121969
- DOI -
- Views 68
- Downloads 0
Sympathetic Activity and Meditation-Gender Based Variability
The current study was done to see the gender variability in the sympathetic activity as a result of Rajyoga Meditation. For this study we selected 50 participants, 27 males and 23 females in age group 25 to 60 years, per...
Effects of the Aqueous Fruit Extract of Solanum macrocarpum Linn. on Hematological Parameters of Chronic Triton-Induced Hyperlipidemic Rats
The dried and pulverized unripe fruit of Solanum macrocarpum (Solanaceae) was soxlet-extracted with distilled water. Studies were then undertaken on the effect of the aqueous extract on hematological parameters and the l...
Study of Possible Colonic Mucosal and Motility Changes in Experimental Acetic Acid Induced Colitis in Male Albino Rats under the effect of Oral Barley Administration in Comparison with Venlafaxine
The current study was conducted on 40 male albino rats subjected to acetic acid 4% induced colitis except the negative control group I .Its scope concerned histological and functional colonic evaluation of pretreatment w...
Effect of Exercise and Quercetin on Obesity Induced Metabolic and Renal Impairments in Albino Rats
Obesity has been described as a state of chronic oxidative stress and inflammatory reaction. This study was carried out to clarify the effect of exercise and antioxidant quercetin on oxidative stress and inflammatory rea...
Effects of Water Crude Leaf Extract of Moringa Oleifera Lam (Moringaceae) on Normotensive Rat Blood Pressure and Isolated Duodenum
Moringa oleifera is used in folk medicine for the treatment of many ailments among which, hypertension and intestinal disorders. The aim of this study was to investigate the hypotensive and/or antihypertensive activity o...