Effects of chronic restraint stress on the expression of PD-1 / PD-L1 in mouse hippocampus, inflammatory response and oxidative stress signaling pathways
Journal Title: Journal of Air Force Medical University - Year 2023, Vol 44, Issue 11
Abstract
Objective To observe the changes in expression level of PD-1 / PD-L1 in the hippocampus of anxiety-like model mice induced by chronic restraint stress (CRS) and the changes of related indexes of inflammatory response and oxidative stress pathways. Methods Twenty-four male C57BL / 6 mice were randomly divided into normal group and model group. The model group was subjected to restraint stress for 2 h every day for 14 d, while the normal group was not treated. The changes in body mass of mice were recorded every 3 d during CRS. The anxiety-like behaviors of mice were evaluated by open-field test and elevated plus maze test. After the behavioral experiments, hippocampal tissues were isolated, and Western blotting was used to detect the expression levels of proteins related to inflammation and oxidative stress in the hippocampus of mice in the two groups. Results ①CRS could induce anxiety-like behaviors and reduce body mass in mice. ②Compared with the normal group, the expressions of IL-1β and NF-κB in the hippocampus of mice in the model group were up-regulated and the expression of IL-1Ra was down-regulated (P < 0. 01), while the expression levels of PD-1 / PD-L1 were significantly decreased (P < 0. 01). ③Nrf2, Keap1 and HO-1 in the model group were significantly lower than those in the normal group (P < 0. 05, P < 0. 01). Conclusion CRS can induce anxiety-like behaviors in mice, inhibit the expression of PD-1/ PD-L1 in hippocampus and regulate the expression of inflammatory and antioxidant pathways in hippocampus.
Authors and Affiliations
SHI Qingqing, LIU Ling, MA Xinxu, ZHANG Tian, WANG Yilin, CHEN Haixia, WANG Huaning
Keywords
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