Experimental Rodent and Human in Vitro-Models for Balanced Activity of Oncogenes and Tumor-Suppressor Genes
Journal Title: Journal of Physiology and Pharmacology Advances - Year 2012, Vol 2, Issue 4
Abstract
Taking in consideration the importance of coordinated oncogenes and tumor-suppressor genes activity in the control of malignancies and death, additional copy of oncogene Dcn1 was inserted in mouse embryonic stem cells (mESCs) by transfection with recombinant DNA-constructs. The noticed absence of phagocyte differentiation of myeloid progenitors, co-cultivated with the so derived mESCs, differed from the data, observed in co-cultivation with malignant cells, containing and non-containing additional copy of tumor-suppressor gene. These data were a proof for preserved non-malignant cell characteristics of the transfected mESCs and were confirmed by their analogy with normal human trophoblasts HTR8/SVneo, immortalized by transfection with recombinant vector SV40. On the other hand, the analogy of malignant rat insulinoma cells RIN-5F with human cervical carcinoma cells HeLa, was also similarly tested. In this connection, the levels of phagocyte differentiation of myeloid progenitors in their co-cultivation with RIN-5F cells, containing additional copy of tumor-suppressor gene SCGN, as well as with HeLa cells with additional copy of tumor-suppressor gene HACE1, were differed of the observed in their co-cultivation with HeLa cells, non-containing additional tumor-suppressor gene. Analogus functions of protein products of both genes SCGN and HACE1 have been indicated as tumor suppressors by their participation in similar cascade mechanisms, such as interactions with cytoskeleton elements, interacting with nuclear proteins. Future studies are necessary in that direction.
Authors and Affiliations
Sainova I , Vavrek I , Pavlova V , Daneva T , Iliev I , Yosifova L , Gardeva E , Nikolova E
Keywords
Related Articles
- EP ID EP114664
- DOI -
- Views 88
- Downloads 0
Preliminary Study of Hypoglycaemic and Hypolipidemic Activity of Aqueous Root Extract of Ricinus Communis in Alloxan-Induced Diabetic Rats
Since diabetes mellitus is a major risk factor that leads to secondary conditions like cardiovascular disease, management of the condition include controlling other risk factors that may lead to secondary conditions in a...
Attenuation of Allodynia and Hyperalgesia by the Leaf Extracts of Clerodendrum Formicarum in Rat Model of Diabetic Neuropathic Pain
Painful neuropathy is the most common and debilitating complication of diabetes and results in allodynia and hyperalgesia. Hyperglycemia clearly plays a key role in the development and progression of diabetic neuropathy....
Effects of Lophira lanceolata and of Three Species of Gardenia Leaves Aqueous Extracts on Blood Glucose and Lipids in Wistar Rat
This study has been performed to evaluate the hypoglycemic, anti-hyperglycemic and hypolipidemic properties of aqueous extracts of Lophira lanceolata, Gardenia aqualla, Gardenia nitida and Gardenia ternifolia, plants use...
Effect of Supplemental Feed Additives on the Performance of Broilers
Poultry farmers are usually desirous of getting their birds to reach market weight within the shortest possible period. While this could be dependent on various factors such as the type or breed of the day-old-chick, the...
The Effect of Silymarin on the Ultra Structural of the Photoreceptor Layer in Streptozotocin-Induced Diabetic Rats
In present study silymarin was tested against damage of photoreceptor layer in streptozotocin-induced diabetic rats. Fifteen adult male wistar rats divided into three groups of five rats each. Diabetes was induced in two...