IN VITRO ANTIPROLIFERATIVE AND IN SILICO ACTIVITY OF RUBIADIN ISOLATED FROM ROOTS OF Rubia Cordifolia
Journal Title: Mintage journal of pharmaceutical and medical sciences - Year 2016, Vol 5, Issue 1
Abstract
Background: Rubia cordifolia Linn.[ Rubiaceae], commonly known as Indian Maddar and Manjistha, is used as medicine for treatment of various ailments in Traditional System of Medicine of India. Purpose: To isolate rubiadin and evaluate in vitro antiproliferative activity and in silico method. Material and Methods: Rubiadin was isolated from the roots of R.cordifolia. Rubiadin was characterized by IR, 1H-NMR, 13C-NMR and Mass spectrum. Standardization of rubiadin was done also by HPLC fingerprinting. In vitro antiproliferative activity was done using HeLa cell lines by MTT assay at different concentrations ranging from 20-100 µg/ml in triplicate and in silico docking studies using enzyme EGFR tyrosine kinase. Results: Fingerprinting of isolated rubiadin were done by HPLC method. The IC50 value was found to be 56.63 ± 0.025 µg/ml in in vitro antiproliferative activity in HeLa cell lines. Rubiadin was subjected to molecular docking studies for the inhibition of the enzyme EGFR tyrosine kinase, which is one of the targets for inhibition of cancer cells. It has shown -7.07 kJ mol-1 binding and -7.12 kJ mol-1 docking energy with two hydrogen bonds. Conclusion: Rubiadin has shown to possess in vitro antiproliferative activity and in silico studies.
Authors and Affiliations
padmaa m paarakh, dileep chandra sreeram, shruthi sd, sujan ganapathy ps, vedamurthy ab
Keywords
Related Articles
- EP ID EP438116
- DOI -
- Views 154
- Downloads 0
A DISCUSSION ON QUALITY CONTROL OF SUPPOSITORIES
Suppository quality control includes physical and chemical aspects of the product. The physical analysis includes visual examination (physical appearance), uniformity of weight, uniformity of texture, melting point, liqu...
A STUDY OF PREDICTABILITY OF BLEEDING RISK IN PEDIATRIC DENGUE INFECTION WITH SIMPLE HAEMATOLOGICAL TESTS
Background and aim: Predicting of bleeding risk in dengue infection with feasible means continues to be important concern. This study evaluates usefulness of basic hematological tests in this respect. Method: This is cro...
STUDY OF FEW TETRALONE DERIVATIVES
Tetralone ring system has drawn an immense attention in the field of drug discovery by virtue of their wide applications as an analgesic, antidepressant, antifungal and antibacterial. The wide range of its therapeutic ap...
DIVERSIFIED EXCIPIENT CHARACTERIZATION OF A BIOCOMPATIBLE EXCIPIENTS
Objective: The main Objective of the present work was polymers obtained from natural origin has great compliance in the Pharmaceutical industry and most suitable for use in formulations. Material Used: Sago is a powdery...
EVALUATION OF ANALGESIC ACTIVITY OF Hibiscus Schizopetalus BY CENTRAL AND PERIPHERAL MODELS
Objective:The present study was aimed to evaluate the central and peripheral analgesic activity of ethanolic extracts of Hibiscus schizopetalus (EEHS) (Mast) Hook (Malvaceae) in Wistar rats. Methods: Rats weighing about...