MULTIBIOSENSOR SYSTEM BASED ON PH-SENSITIVE FIELD-EFFECT TRANSISTORS FOR SIMULTANEOUS DETERMINATION OF GLUCOSE, CREATININE AND UREA
Journal Title: Сенсорна електроніка і мікросистемні технології - Year 2018, Vol 15, Issue 2
Abstract
In this work, the multibiosensor system was developed for the simultaneous determination of glucose, urea and creatinine concentrations. To prepare bioselective elements we used glucose oxidase, recombinant urease, and creatinine deiminase, which were immobilized on the surface of pH-sensitive field-effect transistors by covalent cross-linking with bovine serum albumin using glutaraldehyde. In the frame of research, the possibility of simultaneous function of all bioselective elements in the multibiosensor system was checked. The linear ranges of determination were: urea 0.5 - 10 mM, creatinine - 0.08 - 2 mM, glucose - 0.08 - 1 mM. These values were adequate for the simultaneous determination (the same dilution of sample) of the above metabolites in the blood serum of patients with renal insufficiency. The bioselective elements were separately checked for the presence of substrates cross-effect, and a possibility of their simultaneous work was proven from this viewpoint as well. It was shown that the multibiosensor system is characterized by good reproducibility of responses during continuous operation over one working day. The developed system was tested for evaluation of glucose, urea and creatinine concentrations in the blood serum of patients with renal insufficiency. The obtained results confirmed the optimistic prospects of the developed multibiosensor assay.
Authors and Affiliations
O. O. Soldatkin, S. V. Marchenko, A. L. Kukla, A. S. Pavluchenko, A. P. Soldatkin
Keywords
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- EP ID EP399963
- DOI 10.18524/1815-7459.2018.2.136888
- Views 309
- Downloads 0
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