COMPARATIVE EVALUATION OF A COLORIMETRIC MICROTITRE PLATE ASSAY IN DETERMINING THE MINIMUM INHIBITION CONCENTRATION OF ANTIMICROBIALS AND PRESERVATIVES IN COSMETIC AND TOILETRY FORMULATIONS
Journal Title: International Journal of Pharmaceutical Development & Technology - Year 2015, Vol 5, Issue 2
Abstract
ABSTRACT Preservatives and antimicrobials are commonly used in cosmetic and toiletry products in order to increase the shelf life of the products. Thus the effective concentration of these chemical agents is one of the most critical points in formulation of cosmetics/ toiletries. Though there are some standard tests to determine the efficiency of antimicrobial preservatives in personal care products, no effective method is available that could determine the in situ presence of the antimicrobials and whether the required concentration is achieved within the final product. Personal care products when assayed for their anti-microbial contents by routine methods suffer due to the inherent turbidity of the product, thus the conventional method was miniaturized using a 96 well microtitre plate. An indicator colorimetric assay was developed to overcome the routinely used two step system of determining the MIC and MCB by test tube dilution followed by agar spotting. The methods were compared and assessed to develop a standardized technique for determining the in situ minimum inhibitory concentration of the preservative in cosmetic and toiletry formulations with the micro dilution assay being most accurate followed by micro dilution indicator dye assay followed by the conventional macro dilution assay. Keywords: Antimicrobials, Cosmetic preservatives, Micro dilution assay, Macro dilution assay, Indicator dye assay, 2, 3, 5- Triphenyltetrazolium chloride (tetrazolium salt).
Authors and Affiliations
Z. P. Bhathena 1*& N. J. Desai 2
Keywords
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COMPARATIVE EVALUATION OF A COLORIMETRIC MICROTITRE PLATE ASSAY IN DETERMINING THE MINIMUM INHIBITION CONCENTRATION OF ANTIMICROBIALS AND PRESERVATIVES IN COSMETIC AND TOILETRY FORMULATIONS
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