Preliminary Phytochemical , Chromatographic And Antimicrobial Evaluation Of Ethanol Leaves Extract And Fractions Of Andrographis Paniculata (Burm.F.) Nees Family Acanthaceae
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 5
Abstract
This study is designed to examine the ethanol leaves extract of Andrographis paniculata (Burm.f.) Nees family acanthaceae for its in vitro antimicrobial potential against strains of Streptococcus pneumoniae, Escherisha coli, Pseudomonas aeruginosa and Candida albicans at five concentrations; 200, 100, 50, 25 and 12.5mg/ml respectively by agar well diffusion method to ascertain its folkloric claim to treat various infectious diseases. The extracts showed significant broad spectrum antibacterial activity. The minimum inhibitory concentration of the bacteria ranged between 7mg/mL and 12.5mg/mL while that of Candida albicans was 1.42mg/mL. Phytochemical analysis revealed the presence of terpenoids, tannins, flavonoids, saponins, alkaloids, reducing sugar, steroids, and cardiac glycoside. TLC analysis of the hydrolysed fractions obtained by solvent extraction technique resolved fraction A into three components (Rf = 0.41, 0.46 and 0.82), fraction B into two components (Rf = 0.27 and 0.58), fraction C into two components (Rf = 0.42 and 0.65), fraction D into one component (Rf = 0.87) and fraction E into two components (Rf = 0.48 and 0.76). Distillation of the volatile oil gave oil with the refractive index of 1.3685 and boiling point of 220 – 221oC. These findings explicitly support its traditional claims in treating skin infections, pneumonia, respiratory tract infection, diarrhoea and dysentery.
Authors and Affiliations
T. U Onyekaba, Nwoye David Ifeanyi, Anowi Chinedu Fredrick
Keywords
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- EP ID EP415698
- DOI 10.9790/3008-1305055464.
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