Role of High Performance Liquid Chromatography (HPLC) screening test in Young population to prevent thalassemia and other hemoglobinopathies.
Journal Title: Pathology And Laboratory Medicine - Year 2018, Vol 9, Issue 2
Abstract
Thalassemia is a heterogeneous group of inherited disorders of hemoglobin characterized by reduced or absent production of globin chains.It is the commonest single gene disorder in india.Inherited disorders of Hb due to the structural alteration of globin chain are called hemoglobinopathies. Material and method : We had screened total 1071 healthy students.The tests were performed on an instrument manufactured by BIO RAD laboratories,USA.The instrument, known as BIO RAD 'VARIANT' (beta thalassemia short program) utilizes the principle of high performance liquid chromatography (HPLC) for various hemoglobinopathies and variants as described in instruction manual forthe assay. Result : Out of total 1071 apparently healthy students screened, we had detected 50 cases which displayed abnormal hemoglobin fractions on HPLC.A total of 25cases (2.33%) of beta thalassemia trait were diagnosed followed by 20cases (1.86%) of sickle cell trait and 5cases (0.46%) of HbD Punjab trait were diagnosed. Conclusion : HPLC has been shown to be as an emerging tool which is sensitive, specific and reproducible alternative to electrophoresis. With automation and quantitative power, it appears to be a sensitive and accurate technique with a high degree of precision in the quantification of major and minor,normal and abnormal, hemoglobin variants.
Authors and Affiliations
Niharkumar Chaudhari, Dr. Nidhi Bhatnagar, Mamta Shah, M D Gajjar
Keywords
Related Articles
- EP ID EP516327
- DOI -
- Views 73
- Downloads 0
Effectiveness of cell block technique in diagnostic pathology
Background : One of the constraints of the conventional Fine Needle Aspiration smear is the limited material available for adjuvant diagnostic investigations. This employs the retrieval of small tissue fragments from the...
Follicular Thyroid Carcinoma Presenting With Frontal Bone Metastasis - A Rare Case Report
Follicular thyroid carcinoma (FTC) is the second most common well differentiated malignant tumor after papillary carcinoma amongst all thyroid malignancy, account for 10-20% cases. FTC has high tendency to spread through...
Determination of baseline titre of widal test among healthy individual in Bhavnagar district, Gujarat
Widal test is simple serological investigation for the diagnosis of typhoid fever for that determination of baseline titre among healthy individual in a community required. Present study was supervised to determine the b...
Dengue & Chikungunya fever, re-emerging virus infections: Diagnosed by real time PCR
Background: Dengue Virus (DENV) &Chikungunya Virus (CHIKV), are emerging viral infections, have become a serious health problem in recent years. Re-emeregence of the other arboviral heamorrhagic fever &overlapping signs...
Relapse of typhoid fever – A case study
5-10 % of patients treated with antibiotics experience relapse of typhoid fever after initial recovery. A 9 year child with blood culture confirmed Typhoid fever was treated with inj. Ceftriaxone for 5 days with complete...