STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF CANDESARTAN CILEXETIL AND HYDROCHLOROTHIAZIDE IN BULK AND DOSAGE FORMS
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 5
Abstract
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination of Candesartan cilexetil and Hydrochlorothiazide in pharmaceutical dosage form. The column used was Hypersil BDS C18 (150*4.6mm, 5µ) in isocratic mode, with mobile phase containing phosphate buffer- acetonitrile (55:45) adjusted to pH 4.6 using ortho phosphoric acid was used and injection volume of 20µL, with a flow rate of 1.0ml/min. and effluents were monitored at 244 nm. The retention times of Candesartan cilexetil and hydrochlorothiazide were 2.6 min and 3.6.min, respectively. The linearity for Candesartan cilexetil and Hydrochlorothiazide were in the range of 38.4-86.6 mcg/mL and 30-70 mg/mL respectively with correlation coefficient of r2=0.999 for both. The assay of the proposed method was found to be 99.38% and 99.26%. The recoveries of Candesartan cilexetil and Hydrochlorothiazide were found to be 101.3% and 99.86%, respectively. The % RSD from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Candesartan cilexetil and Hydrochlorothiazide in bulk and in Pharmaceutical dosage form.
Authors and Affiliations
Veeranjaneyulu D, Aneesha A, Nandakishore Agarwal
Keywords
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