STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF CANDESARTAN CILEXETIL AND HYDROCHLOROTHIAZIDE IN BULK AND DOSAGE FORMS
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 5
Abstract
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination of Candesartan cilexetil and Hydrochlorothiazide in pharmaceutical dosage form. The column used was Hypersil BDS C18 (150*4.6mm, 5µ) in isocratic mode, with mobile phase containing phosphate buffer- acetonitrile (55:45) adjusted to pH 4.6 using ortho phosphoric acid was used and injection volume of 20µL, with a flow rate of 1.0ml/min. and effluents were monitored at 244 nm. The retention times of Candesartan cilexetil and hydrochlorothiazide were 2.6 min and 3.6.min, respectively. The linearity for Candesartan cilexetil and Hydrochlorothiazide were in the range of 38.4-86.6 mcg/mL and 30-70 mg/mL respectively with correlation coefficient of r2=0.999 for both. The assay of the proposed method was found to be 99.38% and 99.26%. The recoveries of Candesartan cilexetil and Hydrochlorothiazide were found to be 101.3% and 99.86%, respectively. The % RSD from reproducibility was found to be <2%. The proposed method was statistically evaluated and can be applied for routine quality control analysis of Candesartan cilexetil and Hydrochlorothiazide in bulk and in Pharmaceutical dosage form.
Authors and Affiliations
Veeranjaneyulu D, Aneesha A, Nandakishore Agarwal
Keywords
Related Articles
- EP ID EP33116
- DOI -
- Views 371
- Downloads 0
Solubility enhancement of an antifungal agent by association with dendrimers
Amphotericin B is a polyene antifungal agent which is not freely soluble in water. The clinical use of amphotericin B is limited by nephrotoxicity and poor water solubility, which leads in some cases to permanent renal...
PREPARATION, CHARACTERIZATION AND EVALUATION OF OLMESARTAN MEDOXOMIL-Β CYCLODEXTRIN COMPLEXES
Olmesartan medoxomil is a poor water soluble drug. There are numerous approaches available and reported in literature to enhance the solubility of poor water soluble drugs among which cyclodextrin complexation is predo...
Fabrication, Characterisation and Evaluation of Rivastigmine Loaded Transdermal Films for the Patients Enduring with Alzheimer’s disease
Alzheimer’s disease is the most common type of dementia which makes up 50 – 70% cases. Various cholinesterase inhibitors are used to treat conditions related to dementia. Among many drugs Rivastigmine is most promising...
Development of RP-HPLC method for the estimation of Oseltamivir in pharmaceutical dosage form
A rapid and precise Reverse Phase High Performance Liquid Chromatographic method has been developed for the validated of Oseltamivir, in its pure form as well as in tablet dosage form. Chromatography was carried out on...
Formulation and evaluation of matrix floating tablets of Norfloxacin and Tinidazole combination
Floating drug delivery systems have shown to be of better significance in controlling release rate for drugs having site specific absorption. The present study was an attempt to develop floating tablets Tinidazole and...