Study of the role of animals as the residues of the human hepatitis virus
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2019, Vol 35, Issue
Abstract
Introduction. Interest to the problem of viral hepatitis E has grown in the last decade. In studies conducted in different countries, the hepatitis E virus (HEV) was detected in animals (wild boars, pigs, birds, wild rats, etc.), and the role in the occurrence of acute hepatitis E in humans was demonstrated. Animals are involved in the circulation of the HEV in nature, therefore, hepatitis E is a zoonosis. In countries where hepatitis E was detected and serological studies were conducted among various animal species, it was shown that most number of antibodies were registered in pigs, rabbits, birds, cattle. The goal of the work was to conduct studies on the establishment of the circulation of the HEV in rabbits, domestic and wild pigs in the Republic of Belarus. Materials and methods. To determine the serological markers of HEV infection an enzyme immunoassay was used for anti-VGE detection using the Vector-Best Cytogenesis-E test kit (Novosibirsk). We developed a test kit for detecting antibodies to HEV in the blood serum of pigs and rabbits using the indirect ELISA, where recombinant ORF2 and ORF3 antigens were used. Determination of the IgG-antibodies using conjugate consisted of horseradish peroxidase with solution of Staphylococcus aureus protein. In this study 124 samples of rabbit blood serum, 987 samples of blood serum obtained from domestic pigs and 11 from wild boars were analyzed. Results of research and discussion. Revealed results showed that in domestic pigs from various pig farms antibodies were detected in 26.7% of the examined pigs and in 20% of the examined rabbits. Conclusions and prospects for further research. The circulation of the HEV among some animals in the Republic of Belarus was proved, its approximate scales were determined.
Authors and Affiliations
P. A. Krasochko, P. P. Krasochko, A. P. Kurdeko, S. V. Zvavoronok, V. V. Dovidov, A. A. Arabie, D. S. Borisovets, T. M. Prokopenkova, S. A. Nychyk
Keywords
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- EP ID EP660085
- DOI 10.31073/vet_biotech35-10
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