The antineuroinflammation effects of Nimodipine after severe traumatic brain injury in male rat
Journal Title: International Neuroscience Conference (NEURO-2023) - Year 2023, Vol 4, Issue 1
Abstract
Introduction: Nimodipine is a dihydropyridine calcium channel antagonist that blocks extracellular calcium currents through L-type and voltage-gated calcium channels. The use of calcium channel blockers has been suggested for the prevention or treatment of cerebral vasospasm after acute traumatic brain injury, therefore, in this research project, the role of nimodipine neuron protection in the process of diffuse concussion in rats and its effect on the level of interleukins and we examined the histological changes. Materials and methods: After induction of anesthesia and cannulation in the trachea, 56 Wistar rats underwent diffuse controlled brain injury by Marmarou method, and 30 minutes later, the drug was injected intraperitoneally with different doses and repeated in the following days. In the pre-traumatic times, immediately after recovery from trauma induction, 24, 48 and 72 hours after trauma, Veterinary Coma Scale and Beam Walk and Beam Balance movement and balance tests were taken and recorded from rats. After 72 hours, CSF was collected from Cisterna Magna and used for ELISA test to evaluate the level of interleukins. Rats were killed under deep anesthesia and their brains were removed and fixed in 10% formalin for 48 hours. Staining with hematoxylin and eosin was used. Blood-brain barrier permeability was tested by Evans dye injection after induction of trauma in rats of the respective group. Result: Findings of this study show that brain injury caused by traumatic brain injury causes cerebral edema, destruction of the blood-brain barrier, disturbance of neurological and balance-motor scores of the animal (P <0.0001). It also leads to an increase in interleukin 1 beta and a decrease in interleukin 10 in CSF fluid (P <0.0001). Our findings also showed that nimodipine at doses of 2 mg / kg and 4 mg / kg can reduce these differences compared to the control group (Sham and Intact) (p <0.001). It should be noted that nimodipine was more effective at 4 mg / kg (P <0.0001). Discussion: in this study, it can be found that firstly, dose-dependent nimodipine has neuroprotective effects in the brain and was able to affect the consequences of trauma and reduce cerebral edema, accelerate the improvement of blood-brain barrier and neurological scores and be vistibulomotor function. Second, Histological changes have also been shown to improve. These effects of nimodipine may be due to a decrease in inflammatory interleukins and an increase in anti-inflammatory interleukins.
Authors and Affiliations
Taha Zendehdel, Taha Zendehdel, Ali Siahposht-Khachaki*
Keywords
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