The effects of Minocycline on the neurological and inflammatory factors expression after severe traumatic brain injury in male rats: A molecular and histological study
Journal Title: International Neuroscience Conference (NEURO-2023) - Year 2023, Vol 4, Issue 1
Abstract
Introduction: Traumatic brain injury (TBI) is a very complex disorder that includes different degrees of brain contusion, diffuse axonal damage, haemorrhage, and hypoxia. Minocycline is an antibiotic that acts through anti-inflammatory activities and has neuroprotective effects in the central nervous system. Therefore, in this research project, we investigated the role of neuron protection of minocycline in the process of diffuse concussion in rats, as well as its effect on the level of interleukins and histological changes. Materials and methods: After induction of anesthesia and cannulation in the trachea, Wistar rats were subjected to diffuse controlled concussion using the snake method, and 30 minutes later, the drug was injected intraperitoneally with different doses. It was repeated in the following days. After 72 hours, the brain tissue was collected from the Cisterna Magna and was designed to examine the expression changes of IL-1β, IL-10, Bcl-2 and BAX genes, and using the Real-Time PCR method, the expression changes of the mentioned genes in Brain samples of different groups were evaluated. And the rats were killed under deep anesthesia and their brains were removed and fixed in 10% formalin for 48 hours and used for staining with hematoxilin and eosin. Findings: The findings of this study show that brain injury caused by controlled diffuse trauma causes brain tissue edema and changes in the animal's neurological and balance scores. It also leads to an increase in the expression of interleukin 1 beta and a decrease in interleukin 10 in the brain tissue. Also, our findings showed that minocycline in doses of 22.5 mg/kg and 45 mg/kg can reduce these differences compared to the control group (p<0.001)). It should be noted that minocycline was more effective at a dose of 22.5 mg/kg (P<0.0001). Discussion and conclusion: Based on this study, it can be seen that, firstly, minocycline has neuron protection effects in the brain and has been able to affect the consequences of concussion and reduce cerebral edema, accelerate the improvement of the blood-brain barrier and grades. Be neurological and balanced. Secondly, these effects of minocycline are through the reduction of inflammatory interleukins and the increase of anti-inflammatory interleukins and the effect on their genes.
Authors and Affiliations
Ali Siahposht-khachaki*, Mohammad Rahnama, Mozhgan Abbasi
Keywords
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