The effect of intracerebroventricular injection of ghrelin receptor antagonist on the extinction and reinstatement phases following morphine induce conditioning place preference in male rat: a behavioral and biochemical study
Journal Title: International Neuroscience Conference (NEURO-2023) - Year 2023, Vol 4, Issue 1
Abstract
Introduction: This increases the release of dopamine in the nucleus accumbens, which determines the sensation of pleasure. The ghrelin receptor is located in many areas of the brain associated with pleasure, reward, and motivation. The expression of ghrelin receptor mRNA has been seen in the substantia nigra and VTA, and the location of its receptor in the reward pathway may indicate the role of ghrelin in pleasure and reward. Ghrelin receptors are connected to VTA neurons. The expression of ghrelin receptors in VTA neurons causes the administration of ghrelin in this area to release dopamine. Because the function of the reward system of the brain in reward and drug dependence is well known, therefore, in this study, the effects of intracerebroventricular injection of ghrelin receptor antagonist (JMV2959) on the period Extinction and reinstatement of morphine have been discussed. Material and methods: During the conditioning period, the animals received morphine at a dose of 5 mg/kg of body weight for three days. On the fifth day of the expression phase, they entered the shutdown phase and returned to morphine consumption again. During the blackout period half an hour before taking the conditioning test, different doses of JMV2959 (20, 10 and 5 μg/kg body weight) were received intracerebroventricularlly (ICV) and on the day of return to use after taking a dose of morphine (dose of 1 mg/kg of morphine) and after taking the conditioning behavioral test, the head of the animal is separated under deep anesthesia and the nucleus accumbens is separated according to the Watson-Paxinus atlas and fixed in liquid nitrogen and used for biochemical assay. Results: Our findings showed that daily intracerebroventricular injection of JMV2959 in doses of 5, 10 and 20 micrograms caused a significant reduction in the period of blackout and return to re-use compared to the control group (P<0.0001) and single dose administration. 20 micrograms before the return period caused a decrease in the conditioning score in this period. In the groups treated with JMV2959, biochemical changes (c-Fos) in the area of the nucleus accumbens showed improvement in a dose-dependent manner (P<0.0001). Discussion: These findings show that JMV2959 can be effective in the period of extinction and reinstatement of morphine and possibly useful in forming the basis of addiction.
Authors and Affiliations
Faramarz Shirani-bidabadi, Zahra Mahrooz, Ali Siahposht-Khachaki*
Keywords
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