Use of micronucleus test for screening and monitoring of mutagens. Historical controls
Journal Title: Український журнал сучасних проблем токсикології - Year 2017, Vol 79, Issue 3
Abstract
Introduction. Analysis of mutagenic activity is a required element of toxicological assessment of pesticides. Order to provide higher reliability in assessing the results of experiments, current research data are compared with the data historical controls. Purpose of the study. It is necessary to form a historical control data based on the results obtained during the mutagenicity investigations of generic pesticides in the micronucleus test in the bone marrow of mice in vivo and compare the results with the published data in literature. Materials and Methods. Experimental studies of the mutagenic activity of the test substances in the micronucleus test in polychromatic erythrocytes in the bone marrow were performed by the standard method in compliance with requires of GLP, for young adult mouse species CD1 albino, males. To compare the results of own research we used data published in 2000 by the authors G. Krishna and colleagues. Results and Discussion. In the Laboratory of Experimental Toxicology and Mutagenesis for the period 2015–2016 years we have conducted 28 experiments to create a database of positive and negative controls for this test. The average values of the levels of micronucleated bone marrow polychromatic erythrocytes in our laboratory coincides with the data obtained G. Krishna and colleagues. Conclusions. In the Laboratory of Experimental Toxicology and Mutagenesis we validated and put into practice the micronucleus test in polychromatic erythrocytes in the mice bone marrow in vivo (OECD 474). A database of positive and negative controls was created on the research results of this test. Comparison of the levels of historical control database with the published in the literature showed that the data from our laboratory are within the same distribution. Created by us historical control can be used for reliable evaluation of research results. The levels of Historical Control database indicates the high quality of the conditions of animal housing and feeding and the high genetic quality of animals obtained from SPF nursery of Research Center of Preventive Toxicology for using in this test.
Authors and Affiliations
T. V. Tkachuk, M. G. Prodanchuk
Keywords
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