Medpulse International Journal of Biochemistry

Medpulse International Journal of Biochemistry

Basic info

  • Publisher: MedPulse Publishing Corporation
  • Country of publisher: india
  • Date added to EuroPub: 2019/Nov/07

Subject and more

  • LCC Subject Category:
  • Publisher's keywords:
  • Language of fulltext: english
  • Time from submission to publication: weeks

Publication charges

  • Article Processing Charges (APCs): No
  • Submission charges: No
  • Waiver policy for charges? No

Open access & licensing

  • Type of License:
  • License terms
  • Open Access Statement: No
  • Year open access content began: 2017
  • Does the author retain unrestricted copyright? False
  • Does the author retain publishing rights? False

Best practice polices

  • Permanent article identifier:
  • Content digitally archived in:
  • Deposit policy registered in:

This journal has '176' articles

A study of level of lipid peroxide in IDDM and NIDDM patients

A study of level of lipid peroxide in IDDM and NIDDM patients

Authors: S Sumathi, M Nataraj
Year: 2017, Volume: 1, Number: 2
(0 downloads)
Abstract

Aims and Objectives: To study Level of Lipid peroxide in IDDM and NIDDM patients. Methodology: Participants of the study group were selected from the outpatient’s population of Department of Diabetology, Thanjavur Medical College, Thanjavur. 100 patients were selected for this study. Out of which 50 patients belong to NIDDM and 50 to IDDM group. 50 persons served as healthy control. Serum malondialdehyde was estimated by YAGI Method. Un-paired t-test was used for statistical analysis. Result: The average MDA level (Mean±SD) was significantly higher in DM Group. i.e.4.63±0.35 than Control Group i.e. 3.61± 0.11 respectively (P< 0.0001). The average MDA level (Mean ±SD) was significantly higher in NIDDM i.e. 4.8 ± 0.37 as compared to IDDM i.e. 4.46 ± 0.23 than Control i.e. 3.61±0.11 respectively ( P<0.001). Conclusion: It can be concluded from study that cases on insulin as therapeutic regime (IDDM) had lower mean MDA level as compared to those on oral hypoglycaemics (NIDDM) indicating lesser level of oxidative stress in diabetics on insulin.

Keywords: Lipid peroxide, IDDM, NIDDM, MDA (Malondialdehyde).
A study of correlation between lipid peroxide and lipid profile in diabetic patients

A study of correlation between lipid peroxide and lipid profile in diabetic patients

Authors: S Sumathi, M Natraj
Year: 2017, Volume: 1, Number: 2
(0 downloads)
Abstract

Aims and Objectives: To Study Correlation between Lipid Peroxide and Lipid profile in Diabetic Patients. Methodology: Participants of the study group were selected from the outpatients population of Department of Diabetology, Thanjavur Medical College, Thanjavur. 100 patients were selected for this study. Out of which 50 patients belong to NIDDM and 50 to IDDM group. 50 persons served as healthy control. Un-paired t-test was used for statistical analysis. Results: significantly raised level of Total Cholesterol, TGL, LDL,VLDL and decreased HDL in Diabetic as compared to Controls (P<0.0001). There was very good positive correlation between MDA and Cholesterol, TGL, LDL, VLDL in both NIDDM and IDDM cases. There was negative correlation between MDA and HDL in NIDDM and IDDM cases. Conclusion: From our study it can be concluded that, there was very good positive correlation between MDA and Cholesterol, TGL, LDL, VLDL in both NIDDM and IDDM cases. There was negative correlation between MDA and HDL in NIDDM and IDDM cases.

Keywords: Lipid peroxide, IDDM, NIDDM, MDA (Malondialdehyde), Lipid Profile.
Role of 25 hydroxy Vitamin-D in parkinson’s disease

Role of 25 hydroxy Vitamin-D in parkinson’s disease

Authors: Yogesh Krishna Sahay, Madhu Sinha
Year: 2017, Volume: 3, Number: 1
(0 downloads)
Abstract

Abstract Problem statement: Parkinson disease is the second most prevalent neurodegenerative disease worldwide. In the initial stage of the disease, the most obvious symptoms are movement related, which include tremors, rigidity, slowness of movement and difficulty with walking and gait. Behavioural problems and dementia also appears in advanced stage of the disease. Depression is the most common psychiatric symptom and often associated with emotional disorder and sleep disturbances. The disease is common in older and mostly occur after 50 years of age. Methods: It was observational, and case control study to find out that vitamin-D could be regarded as a predictive bio-marker for severity of Parkinson’s Disease. A direct interview of the eligible study participants were carried out treatment sheets were reviewed and after duly explaining the procedures in their local languages. Detailed records of demographic and clinical features were noted in case record proforma. History of name, age sex, religion occupation symptom, duration, history of recurrence etc. was recorded. Patients with PD attended in the Medicine OPD were selected as cases. Total sample size was 30. All subjects was above the age of 60 years The period of the study was one (01) Year beginning from2015 to2016. Results: All cases and control are age match α = 30. Control groups were selected from the community. Fasting glucose level of control and case where within biological reference intervals, mean control value 88.22 ± 13.9 mean case 100.45 ± 8.79. The result is statistically significant p value- <001. Serum Calcium levels of both case and control group are within biological reference interval. Mean value in PD 8.45, SD ± 2.21 and for control 8.83 SD ± 0.49, p Value – <0.3999. It was statistically not significant. Serum level of Vitamin-D in Parkinson Disease (16.51 ± 3.08) is lower in comparison to control group (25.92 ± 3.96) p Value- 0.000. The result was statistically significant control group are within insufficiency range (20 – 29 ng/ml). Conclusion: All patients of our study showed a deficient level (less than 20 ng/ml) of vitamin-D. The control group surprisingly had vitamin-D level in insufficient range (20 – 29 ng/ml). The reason for this insufficient level of Vitamin-D may be searched for in future with large number of sample size.

Keywords: Parkinson’s Disease, neurodegenerative Disease, Hypokinetic rigid syndrome.
Levels of folic acid, vitamin B12 and  Malondialdehyde in stroke patients

Levels of folic acid, vitamin B12 and Malondialdehyde in stroke patients

Authors: Kamalakar Mane, Shilpa Asegaonkar, Arvind Gaikwad, Mahendra Kamble
Year: 2017, Volume: 3, Number: 1
(0 downloads)
Abstract

Background: Stroke is a major health problem in elderly population, however there remains a significant subset of younger patients with ischemic stroke in whom conventional vascular risk factors play a smaller role. Folic acid and vitamin B12 deficiency may cause incr atherosclerosis of cerebral arteries at young age. Oxidative stress characterized by increased generation of oxygen free radicals results in generation of lipid peroxidation product like acute ischemic stroke. Aims and Objectives: MDA in patients of stroke in young with age and sex matched controls. Government Medical College, Aurangabad. we studied 50 cases of stroke in young who were free from conventional vascular risk factors like diabetes mellitus, hypertension, hypercholesterolemia and cigarette smoking with 5 sex matched controls were selected. Their fasting serum folic acid, vitamin B12 levels by chemiluminescence method and serum Malondialdehyde level by Thiobarbituric acid method were determined. serum folic acid and vitamin B12 was significantly decreased in patients of stroke in young compared to controls with p value of <0.0307 and <0.0232 respectively. The serum MDA levels was very highly significant [p<0.001] in patients of stroke in young than in controls. deficiency play as an important independent risk factor for stroke in young patients in factor. The serum MDA was significantly raised

Keywords: Ischemic stroke, folic acid, vitamin B12, vegetarians, oxidative stress, MDA.
A study of glycemic status and lipid profile of obese and non obese type II diabetic patients

A study of glycemic status and lipid profile of obese and non obese type II diabetic patients

Authors:
Year: 2017, Volume: 3, Number: 1
(0 downloads)
Abstract

Background: Diabetes mellitus is a group of metabolic disease characterized by hyperglycaemia resulting from defects in insulin secretion, insulin action, or both. Committee and informed consents were obtained from all participants. 48 (males =16, females=32) known Type 2 diabetic patients aged 35-55 yrs on oral hypoglycemic drugs were Selected in diabetic out care hospital. Independent ‘t’ test was employed to compare groups and p value less than 0.05 was considered significant. Statistical analysis was performed using SPSS software. groups. Group 1 with BMI ≥ 23; Group 2 with BMI ≤ 23. between obese and non-obese groups. Serum insulin, HbA was no significant difference in fasting plasma glucose and lipid profile parameters between obese and non Conclusion: The findings of our study reveal that there

Keywords: Glycemic Status, Lipid Profile, Type II Diabetes, HbA1C, HOMA-IR
Correlation between thyroid profile and liver function tests in pregnancy

Correlation between thyroid profile and liver function tests in pregnancy

Authors: Madhu Sinha, Yogesh Krishna Sahay
Year: 2017, Volume: 3, Number: 1
(0 downloads)
Abstract

Problem Statement: Liver has a key role in thyroid hormones metabolism and their serum level is very important for normal hepatic function and Bilirubin metabolism. Besides the associations between thyroid and liver disease of an autoimmune nature, such as primary biliary c liver injuries and biochemical test abnormalities. The pregnant woman experiences physiological changes to support fetal growth and development. During pregnancy, the serum estrog a maximum during the third trimester. in the dept. of Gyne. and Obst. Anugrah Narayan Magadh Medical College, Gaya. Th 2015 to Oct 2016. 50 pregnant women were included in the study after following the exclusion and inclusion criteria. Normal healthy pregnant women between 21 the biochemical parameters were performed in the department of Biochemistry, Anugrah Narayan Magadh Medical College, Gaya. Results: In this study we observed a normal Fasting blood glucose level in both case and control group. Mean value of cases has been observed as 91.02 (SD±16.04) as compared to controls showing mean value 79.50 (SD±7.98). The result is statistically significant (p Value < 0.000). We found serum Bilirubin level in both case and control group mean value of cases has been obser 0.77(SD± 0.18). The result is statistically not significant (p Value < 0.142). TSH level in both case and control group mean value of cases has been observed as 4.61 (SD± 1.04) as compared t The result is statistically significant (p Value < 0.005).

Keywords: Pregnancy, Hypothyroidism, Thyrotoxicosis, Biliary cirrhosis.
Study of protein metabolism associated with diabetes mellitus

Study of protein metabolism associated with diabetes mellitus

Authors: Kamalakar Mane, Shilpa Asegaonkar, Arvind Gaikwad, Mahendra Kamble
Year: 2017, Volume: 3, Number: 1
(0 downloads)
Abstract

Background: Diabetes mellitus (DM) is ametabolic disease affecting mainly carbohydrate metabolismcon sequenttothis, lipid and protein metabolism area is affected. It metabolism by increasing the cir intends to understand the role of proteins and serum participants of which 50 were fresh lydetected diabetic fulfilled inclusion criteria. Blood samples were drawn under as eptic Necessary investigations were carried out and values were tabulated for ca evaluation. Results: Serum amino acid levels increased significantly (p<0.001) from hemoglobin levels increased significantly (p<0.001) from5.12 (baseline) to11.03. Total (P<0.327) from 7.52 to 5.26. Albumin from 1.44 (baseline) to 1.89. A/Gratio (p<0.791) from 22.17 (baseline) to 20.32 and ALT which showed small effect. increase in circulating amino Total protein, Albumin, A/Gratio, The mechanism and clinical significance

Keywords: Diabetes mellitus, Aminoacids, Glycated hemoglobin, Amino acid Pool.
Urinary albumin creatinine ratio (ACR)- A comparative study on two different instruments

Urinary albumin creatinine ratio (ACR)- A comparative study on two different instruments

Authors: Luca Van Rooy, Vasudha K C, Vanitha Gowda M N, Radhika K
Year: 2017, Volume: 3, Number: 2
(0 downloads)
Abstract

Aim: The aim of this study was to auto analyser. Study Design: years, that was received in the Biochemistry laboratory, M S microalbumin /urinary albumin Creatinine ratio was collected. Urinary ACR was estimated using an auto analyser, cobas 6000. 55 of these urine samples that had values for ACR in the normo albuminur estimated for urinary ACR using colorimetric kits. descriptive statistics. The intraclass correlation and Bland Altman plot was used to assess the agre results obtained from the two instruments. confidence interval of 95 % having a highly significant P value of <0.001 for urinary ACR was 87%. Urinary ACR measurement using a colorimetric kit may be a cost effective method to screen for micro albuminuria.

Keywords: Colorimetry, microalbuminuria, urinary ACR, urinary creatinine
Correlation of 24-hour urinary protein, urinary protein: Creatinine ratio and HBA1c in type 2 diabetes with and without nephropathy

Correlation of 24-hour urinary protein, urinary protein: Creatinine ratio and HBA1c in type 2 diabetes with and without nephropathy

Authors: S Mohammed, F Nazki
Year: 2017, Volume: 3, Number: 2
(0 downloads)
Abstract

Background: The leading cause of morbidity and premature mortality in diabetics is nephropathy. closely associated with renal involvement in diabetes. Objectives: To estimate serum levels of HbA1c, 24 ratio in Type 2 diabetics with and without nephropathy. urinary protein and HbA1c levels in these groups. department of Biochemistry, Owaisi Hospital, from October 2016 to May 2017 on 103 diabetes patients comprising of 41 diabetics without ephropathy (Group A) and 62 Type 2 diabetes subjects with nephropathy (Group B) parameters were estimated: Fasting blood glucose, Serum creatinine, HbA1c Results show that the mean values of fasting blood protein to creatinine ratio are significantly increased in Group B when compared to Group A and these parameters are further increased in diabetic subjects with P: C ratio protein: creatinine ratio levels are significantly increased (p< 0.001) with proteinuria more than the physiological range (P: C ratio 0.2) than within physiologicalrange (P: C < positively with high gnificance.HbA1c levels correlated positively with both P:C ratio and 24 levels. Conclusion: This study suggests estimating HbA1c as an indicator of gl method for early diagnosis and intervention for diabetic nephropathy.

Keywords: Type 2 Diabetes; HbA1c; protein: creatinine ratio; 24- hour urinary protein.
Cord blood bilirubin as a predictor of neonatal hyperbilirubinaemia

Cord blood bilirubin as a predictor of neonatal hyperbilirubinaemia

Authors: H N Khan, A M Siddiqui, Sujata M Gaikwad, Amol R Shinde
Year: 2017, Volume: 3, Number: 2
(0 downloads)
Abstract

Hyperbilirubinaemia is a universal problem as it affects about 60% of full term first week of life. The aim of this study is to evaluate the predictive value of umbilical cord bilirubin in for subsequent hyperbilirubinemia, in full full term (FT) and 35 Late preterm (late PT) significantly higher as compared to neonatal hyperbilirubinemia and allowing safe postnatal Hospital discharge.

Keywords: Hyperbilirubinaemia, Full term, Late preterm
Study of HbA1c and lipid profile in Type II diabetes mellitus

Study of HbA1c and lipid profile in Type II diabetes mellitus

Authors: S A Patel, H N Khan, A M Siddiqui, W A Shaikh
Year: 2017, Volume: 3, Number: 2
(0 downloads)
Abstract

Background: Dyslipidaemia and poorly controlled blood glucose levels as indicated by HbA1c Type II diabetes mellitus. Therefore study of HbA1c and lipid profile in these patients could be helpful in prognostic aspects. Aim: To study HbA1c and lipid profile in type II Diabetes mellitus patients. 50 already diagnosed type II diabetes mellitus patients and 50 controls were selected. We estimated and compared HbA1c and lipid profile in them. the statistical significance. Result: HDL-C concentration decreased in type II diabetes meliitus as compared to that of control. mellitus is associated with poor glycaemic control and dyslipidaemia

Keywords: HbA1c and lipid profile
Total proteins, albumin and HBA1c in type 2 diabetes mellitus

Total proteins, albumin and HBA1c in type 2 diabetes mellitus

Authors: F A Nazki, A Syyeda, S Mohammed
Year: 2017, Volume: 3, Number: 3
(0 downloads)
Abstract

Type 2 diabetes mellitus is a metabolic disorder characterized by insulin resistance [affecting carbohydrate, lipid and protein metabolism]. Glycation of proteins, including hemoglobin and albumin have been implicated in comp diabetes. The aim of the present study was to evaluate the association between variations in the levels of albumin and total proteins with glycated hemoglobin [HbA1c]. Present study involved 408 participants who visited Owaisi Hospital and Research Center, Hyderabad from November 2015 to June 2017. 204 were freshly detected diabetic patients and 204, age and sex matched healthy controls. Fasting blood glucose levels, HbA1c, plasma total proteins and plasma albumin were estimated. The objective diagnosed cases of Type 2 Diabetes Mellitus. HbA1c levels increased (p<0.001) from 4.90 ± 0.53 in controls to 9.02 ± 2.06 in cases, plasma albumin levels were decreased (P<0. plasma total protein levels were increased from 6.8±0.81 in controls to 7.31±0.93 [p< 0.0013*] in cases. Globulin levels increased (P<0.50) from 1. 68 ± 0.29 in controls to 1.74 ± 0.57 in cases. 0.43 in controls to 1.84± 0.36 in cases. Glycation accelerates albumin degradation via increasing catabolic rate and decreasing protein half-life, and competes with other proteins for glycation. Our study sho between HbA1c and serum albumin. Freshly detected diabetic patients were associated with increased total proteins and decreased A/G ratio. We observed in our study that albumin levels may be associated inversely with HbA1c values results may lead us to study the ways in which glucose and proteins may interact in a newer approach and could aid in understanding hyperglycemia and its estimation.

Keywords: Albumin, HbA1c, total proteins, Type 2 Diabetes mellitus.
Modified method for partial purification of human blood clotting factor VIII from fresh frozen plasma

Modified method for partial purification of human blood clotting factor VIII from fresh frozen plasma

Authors:
Year: 2017, Volume: 3, Number: 3
(0 downloads)
Abstract

Human blood is an irreplaceable commodity. Its proteinaceous structure, the compatibility and others. Factor VIII to the haemophilic patient provides need to make available Clotting Factor VIII from freeze dried fresh plasma with Factor VIII of coagulation has longer shelf life (2 years) and can be introduced to the patient with less interference of immunological complications. The present study established cryoprecipitate - rich in Coagulation Factor VIII without significant loss of activity. The gradual treatment of change in temperature followed by lyophilisation produced enriched fraction. Thus the Coagulation Factor for infusion to the required haemophilic patient readily.

Keywords: Cryoprecipitate, Stages of lyophilisation, Blood Safety and Quality regulations (BSQR), Clotting Factor VIII
Bilirubin content and dielectrophoresis of erythrocytes of patients suffering from jaundice

Bilirubin content and dielectrophoresis of erythrocytes of patients suffering from jaundice

Authors: Farha Isna, Raisa Faheem, Kaleem Ahmed Jaleeli
Year: 2017, Volume: 3, Number: 3
(0 downloads)
Abstract

The paper reports the dielectrophoretic behaviour of erythrocytes of blood of the patients suffering from jaundice. The erythrocytes suspended in isotonic solution were subjected to geometry. For this purpose, a pin dielectrophoretic collection rate (DCR) and threshold voltage (V concentration, frequency and voltage of applied field for normal and diseased blood. The study reveals significant differences in dielectrophoretic collection rate and threshold voltage of erythrocytes of diseased patients, when compared with that of healthy persons. The relations between DCR or V

Keywords: Billirubin, Erythrocytes, Dielectrophoretic collection rate and Threshold Voltage
Study of oxidative stress and uric acid in preeclampsia

Study of oxidative stress and uric acid in preeclampsia

Authors: Mahendra G Dhabe, Mukund R Mogarekar, Choudhari S C, Chanchal C Gujrathi
Year: 2017, Volume: 3, Number: 3
(0 downloads)
Abstract

Aims: This study is aimed to evaluate malondialdehyde levels, serum uric acid levels and serum superoxide dismutase activities were evaluated i women with preeclamosia (Group I) Results: In preeclamptic group lipid peroxidation product malondialdehyde (MDA) (10.45 ± 5.65 v/s3.65 ± 0.72nmol/l) is significantly increased than in normotensive pregnant (control group). The serum superoxide dismutase activity (1.08 ± 0.36 v/s 2.70 ± 0.70 u/ml ) is significantly decreased in preeclamptic group than in controls while uric acid level (5.82 ± 2.47 v/s 3.20 ± 0.65mg/dl) is significantly higher in cases preeclamptic women Conclusion: Increased malondialdehyde and decreased superoxide activity with higher dysfunction are contributing for oxidativ

Keywords: Preeclampsia, malondialdehyde (MDA), antioxidant, superoxide dismutase (SOD), uric acid

About Europub

EuroPub is a comprehensive, multipurpose database covering scholarly literature, with indexed records from active, authoritative journals, and indexes articles from journals all over the world. The result is an exhaustive database that assists research in every field. Easy access to a vast database at one place, reduces searching and data reviewing time considerably and helps authors in preparing new articles to a great extent. EuroPub aims at increasing the visibility of open access scholarly journals, thereby promoting their increased usage and impact.