Analiza częstości występowania polimorfizmu C421A genu ABCG2 w przypadkach raków żołądka w regionie łódzkim
Journal Title: Folia Medica Lodziensia - Year 2014, Vol 41, Issue 1
Abstract
Introduction: In the present thesis the association between the variants of C421A polymorphism of ABCG2 gene and the risk of gastric cancer development were searched. The occurrence of polymorphic variant 421 C > A leads to the weakening of the activity of BCRP protein which is encoded by the ABCG2 gene. Due to the presence of BCRP protein in the gastrointestinal tract and its protective role the reduction of the amount of BCRP protein which is the result of the tested polymorphism may lead to the increased predisposition to cancer development. The main aim of this study was to compare the frequency of C421A polymorphism genotypes between the examined and the control groups. Material and methods: The material for the research consisted of DNA isolated from 19 scraps of the tumor tissue taken from patients with gastric cancer and 44 samples of peripheral blood collected from healthy individuals. DNA was extracted from the tested materials by microcolumn-method. Then the obtained samples of DNA were subjected to polymerase chain reaction (PCR) to amplify the ABCG2 gene fragment. To determine C421A polymorphism genotypes ABCG2 gene fragments amplified by PCR the were analyzed by RFLP method using an digestive enzyme MseI. Results: All the examined patients irrespectively of gender, age and clinical stage showed the presence of genotype C/C, which is homozygous wild for C421A polymorphism. All 44 persons from the control group also showed the presence of genotype C/C. Conclusions: No differences in the occurrence of C421A genotypes between the study group and the control group were found. In both the groups 100% of the results for the C/C genotype were obtained. Due to the presence of only one genotype (C/C) for the C421A polymorphism in all the patients no relationship between the occurrence of C421A genotypes and tumor stage, age and gender of the examined patients was evaluated. Although no correlation between the C421A polymorphism and the susceptibility to the development of gastric cancer was found the exclusion of the participation of BCRP protein in the pathogenesis of this disease is possible only after the increase of the number of analyses on larger groups of respondents.
Authors and Affiliations
Ewa Balcerczak, Aleksandra Sałagacka, Marta Żebrowska, Agnieszka Wosiak
Keywords
Related Articles
- EP ID EP169153
- DOI -
- Views 75
- Downloads 0
Analysis of polymorphisms / mutations of PTEN, CDKN2A, TP53 genes and of hMSH6 gene in endometrial hyperplasia and carcinoma
Endometrial cancer belongs to the most frequent malignancies in the female genital organs with a growing incidence trend. The molecular changes, which are specific for particular stages of neoplastic transformation or th...
Przydatność kliniczna i diagnostyczna oznaczeń D–dimeru w różnych stanach chorobowych
There are many methods dedicated to assessing D–dimer. They are divided into two categories: qualitative and quantitative methods. All the methods are based on immunochemical reaction with the use of monoclonal antibodie...
Czy „cichy” polimorfizm pojedynczego nukleotydu (SNP) C1236T genu ABCB1 jest związany z predyspozycją do rozwoju depresji i skutecznością jej leczenia? - badanie wstępne
Introduction: According to the World Health Organization about 350 million people around the world are affected by depression. Despite the high prevalence of this disease the mechanism of depression origination as well a...
Rola mesalazyny w chemoprewencji raka jelita grubego
Colorectal cancer (CRC) is the fourth most commonly diagnosed cancer in females and the third in males. Every year there are more than a million new cases of colorectal cancer and more than six hundred thousand patients...
Udział zaburzeń szlaku FGF/FGFR w transformacji nowotworowej piersi, jajnika oraz błony śluzowej trzonu macicy
Fibroblast growth factors (FGFs) and their receptors (FGFRs) are the members of a large protein family involved in the regulation of many cellular processes which are essential for proper functioning of cells. It was fou...