Analytical method development and validation for the simultaneous estimation of Rabeprazole sodium and Itopride hydrochloride in bulk and pharmaceutical dosage forms by RP-HPLC
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 6
Abstract
The present investigation describes about a simple, economic, selective, accurate, precise reverse phase high performance liquid chromatographic method for the simultaneous estimation of Rabeprazole sodium and Itopride hydrochloride in pure and pharmaceutical dosage forms. Rabeprazole and Itopride were well separated using a Thermohypersil ODS C18 column of dimension 250 × 4.6, 5µm and Mobile phase consisting of Sodium dihydrogen orthophosphate:Acetonitrile ( pH-5.8) in the ratio of 60:40v/v at the flow rate 1 ml/min and the detection was carried out at 209nm. The Retention time for Rabeprazole and Itopride were found to be 1.950, 5.027 respectively. The developed method was validated for recovery, specificity, precision, accuracy, linearity according to ICH guidelines. The method was successfully applied to Rabeprazole sodium and Itopride hydrochloride combination pharmaceutical dosage form.
Authors and Affiliations
Syed Shaheda, Nanda Kishore Agarwal
Keywords
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- EP ID EP33160
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