Antiradical activities of Salvia officinalis and Viscum album L. extracts concentrated by ultrafiltration process
Journal Title: Acta Scientiarum Polonorum Technologia Alimentaria - Year 2009, Vol 8, Issue 3
Abstract
Background. In the present study the antioxidant properties were investigated of the medicinal plants Salvia officinalis L. (Labiaceae family) and Viscum album L. (Loranthaceae), both of them known for a long time as a remedy in the traditional medicine. The aimof this study was to prove the efficiency of ultrafiltration process for the concentrationof herbs extracts and to evaluate the concentrate’s antioxidant activity.Material and methods. The extracts were prepared by maceration, using different solvents. After filtering the extract through Isolab quantitative filter paper “medium”, each of the filtrates was processed by microfiltration (MF; Millipore filters with 45 μm), followed by ultrafiltration (UF). The regenerated cellulose (Millipore), polysulfone and polyacrylonitrile ultrafiltration membranes were used in the experiment. The initial extracts and samples of permeate and retentate after ultrafiltration of extracts have been characterized by determination of the protein total and total phenolic content. Standard methods like ABTS and DPPH assay are used to measure the antioxidant activity.Results. For the three types of tested membranes: Millipore, PSF and PAN, PAN membrane proves to have the greatest efficiency since it shows the highest permeate flux and the greatest retention degree for bioactive compounds. The concentrated extracts obtained after ultrafiltration with polyacrylonitrile membrane had the strongest scavenging activity for all extracts.Conclusions. The results of this study has revealed that the concentrated extracts havea very high radical scavenging activity (TEAC values for sage hydro-alcoholic concentrated extracts in range 351.87-479.04 μmol Trolox/mL extract and for mistletoe concentrated extract E2 in range 345.14-426.18 μmol Trolox/mL extract; the DPPH inhibition values was over 85% for S. officinalis concentrated extracts and ranges between 66.2% and 88.2% DPPH inhibition for V. album concentrated), therefore can be considered as a good source for further medicinal applications.
Authors and Affiliations
Gabriela Paun Roman, Elena Neagu, Gabriel Lucian Radu
Keywords
Related Articles
- EP ID EP50912
- DOI -
- Views 146
- Downloads 0
Wartość rzeźna i jakość mięsa kulinarnego bydła typu mleczno-mięsnego oraz mięsnego użytkowanego w Polsce
Celem artykułu było porównanie wartości rzeźnej oraz właściwości mięsa bydła najczęściej użytkowanego w Polsce, w kierunku mleczno-mięsnym, z rzadziej spotykanymi rasami mięsnymi, z których uzyskuje się wysokiej jakości...
Właściwości fizykochemiczne i antyoksydacyjne wybranych polskich win gronowych i owocowych
Celem pracy była ocena parametrów enologicznych oraz właściwości antyoksydacyjnych wybranych polskich win gronowych i owocowych. Do badań zostały wykorzystane wina pochodzące z winnicy Golesz na Podkarpaciu oraz komercyj...
The effect of quercetin, chlorogenic acid and epigallocatechin on proliferation of Caco-2 cells
Dietary polyphenols are considered beneficial because of their potential protective role in the pathogenesis of chronic diseases associated to oxidative stress. However, many of these effects may depend on the concentrat...
Utrzymanie jakości owocników Pleurotus ostreatus przez pakowanie w atmosferze modyfikowanej
Wprowadzenie. Grzyby należą do łatwo psujących się towarów. Pakowanie w atmosferze zmodyfikowanej (MAP) jest metodą przedłużania trwałości owocników, pozwalającą zachować cechy świeżego surowca. Celem badania była ocena...
Effect of lacto-ovo-vegetarian diet on serum vitamin B12 concentrations – five-year prospective study
Background. Avoiding of meat is the basic principle of vegetarian diet with several variants existing.In the present study we have focused on lacto-ovo-vegetarian (LOV) diet which includes dairy products and eggs. We hav...